| 摘要 |
PROBLEM TO BE SOLVED: To provide novel and surprisingly effective methods for the detection of nucleic acids, comprising nucleic acid amplification using base-modified deoxynucleoside 5'-triphosphates (dNTPs).SOLUTION: Particular aspects relate to methods for enhancing hybridization properties of oligonucleotide primers and probes in assays detecting nucleic acids. These methods comprise amplifying target DNAs in the presence of base-modified duplex-stabilizing deoxyribonucleoside 5'-triphosphates to provide for amplified target DNAs, and thereby considerably improving performance of the detection assays. The disclosed methods can increase the reaction temperature in PCR-based detection systems, or can reduce the length of the oligonucleotide primers and probes. |
