Negative Regulator of the Abiotic Stress Response

摘要

A small heat shock protein, AsHSP17, that has been cloned from creeping bentgrass (Agrostis stolonifera) is described. Transgenic materials incorporating the nucleic acid encoding the small heat shock protein are also described. Also described are methods for modulating a plant's resistance to abiotic stress via manipulation of the expression of the small heat shock protein. For instance, the small heat shock protein can be utilized as a negative regulator of plant response to adverse environmental stresses.

主权项

1. An isolated nucleic acid molecule comprising one of the following:
a) a synthetic polynucleotide encoding a small heat shock protein as set forth in SEQ ID NO: 1; b) a synthetic polynucleotide encoding a functional equivalent of SEQ ID NO: 1; c) a polynucleotide that comprises the nucleic acid sequence as set forth in SEQ ID NO: 2; d) a polynucleotide that comprises a nucleic acid sequence that is complementary to SEQ ID NO: 2; e) a polynucleotide that hybridizes to SEQ ID NO: 2 under one of the following conditions:
(i) 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM ethylenediamine tetraacetic acid (EDTA) at 50° C. with a final wash in 2× standard saline citrate (SSC), 0.1% SDS at 50° C.;(ii) 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 1×SSC, 0.1% SDS at 50° C.;(iii) 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 0.5×SSC, 0.1% SDS at 50° C.;(iv) 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 0.1×SSC, 0.1% SDS at 50° C.; and(v) 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 0.1×SSC, 0.1% SDS at 65° C.