| 发明名称 | Modalities for the treatment of degenerative diseases of the retina | ||
| 摘要 | This invention relates to methods for improved cell-based therapies for retinal degeneration and for differentiating human embryonic stem cells and human embryo-derived into retinal pigment epithelium (RPE) cells and other retinal progenitor cells. | ||
| 申请公布号 | US9193950(B2) | 申请公布日期 | 2015.11.24 |
| 申请号 | US201414227305 | 申请日期 | 2014.03.27 |
| 申请人 | Ocata Therapeutics, Inc. | 发明人 | Klimanskaya Irina V.;Lanza Robert P. |
| 分类号 | C12N5/079;A61K35/54;A61K35/44;C12N5/0793;A61K35/12;C12N5/071;A61P27/02;A61K35/30;A61K35/36;A61K35/545 | 主分类号 | C12N5/079 |
| 代理机构 | Wolf, Greenfield & Sacks, P.C. | 代理人 | Wolf, Greenfield & Sacks, P.C. |
| 主权项 | 1. A method for the differentiation of human embryonic stem (hES) cells, said method comprising: a) culturing a cluster of cells, produced from hES cells, for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm, under conditions that do not maintain stem cells in an undifferentiated state, wherein the hES cells are stem cells that express Oct-4, alkaline phosphatase, SSEA-3, SSEA-4, TRA-I-60 and TRA-I-81; and b) isolating and culturing the pigmented cells under adherent conditions to form a population of cells that expresses bestrophin, CRALBP, PEDF1 and RPE65. | ||
| 地址 | Marlborough MA US | ||