Method of mixing fluids by coalescence of multiple emulsions

摘要

System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions.

主权项

1. A method of sample analysis, comprising:
obtaining a plurality of compound droplets each including at least one sample-containing droplet, at least one reagent-containing droplet, and a barrier fluid that separates the at least one sample-containing droplet from the at least one reagent-containing droplet; heating compound droplets to induce fusion of at least one of the sample-containing droplet(s) with at least one of the reagent-containing droplet(s) within individual compound droplets to form fused droplets that combine sample and reagent to create assay mixtures for performing an assay; collecting data from a plurality of the fused droplets; and determining a result of the assay based on the collected data.