Guided slicing system for obtaining histological samples and methods thereof

摘要

Systems and methods for providing guided slicing of histological samples. The samples are acquired, spacially labeled with a fiducial marker, and imaged with a scanning system. The images are analyzed, either manually, semi-automatically or automatically, and likely locations of pathologies are identified. A slicing program is then generated and the sample sliced according to the slicing program, thereby ensuring that likely locations of pathologies are analyzed.

主权项

1. A method of guided slicing for obtaining histological specimens, comprising steps of:
a. extracting a sample from an animal; b. spatially labeling said sample by a fiducial marker; c. acquiring ex vivo images of said sample, d. virtually slicing said image, thereby defining, according to the fiducially marked images, N computer-retrievable three-dimensional image slices [3DISs], N is an integer greater than or equal to 2; e. preparing a slicing program of suspected computer-retrievable three-dimensional image slices (SISs) from said N 3DISs; and f. physically slicing said sample according to said SISs; wherein said step of preparing a slicing program comprises a step of defining from said N 3DISs one or more first SISs and one or more second SISs, wherein the slicing plane of said first SISs and the slicing plane of said second SISs are oriented in a non-parallel manner.