| 摘要 |
FIELD: biotechnologies.SUBSTANCE: bacteriorhodopsin obtaining method provides for growth in a fermenter of Halobacterium salinarum VKPM V-11953 strain during six days with further separation of biomass by centrifugation and its destruction at incubation in distilled water during one night at constant stirring. The deposit is separated by centrifugation so that a supernatant is obtained. The obtained supernatant is centrifuged and rejected. Distilled water is added to the obtained deposit in the same volume; the deposit is suspended in water for 3-4 times. After that, the deposit is applied onto a chromatographic column with silica gel based on 200 optic units in an application specimen at wave length of 280 nm per 100 ml of silica gel. After elution is completed, specimens are taken with the ratio of optic density of the solution at wave length of 280 nm to optic density of the solution at wave length of 570 nm (D280/D570) of less than 2.5.EFFECT: invention allows increasing yield of bacteriorhodopsin.1 tbl, 1 ex |
