METHOD TO DETECT AVAILABILITY OF BACTERIA Escherichia coli O157:H7 IN BIOLOGICAL AND FOOD SAMPLES BASED ON IMMUNODETECTION COUPLED WITH POLYMERASE CHAIN REACTION

摘要

FIELD: biotechnologies.SUBSTANCE: method is proposed to detect availability of bacteria Escherichia coli of strain O157:H7 in biological fluids, environment and food, including disintegration of bacteria with fermentative treatment and lysis by a non-ionic detergent, adsorption on paramagnetic particles with the help of a specific monoclonal antibody, detection of antigen of bacteria E.coli O157:H7 with the help of a specific biotin-modified antibody, coupling of the produced complex with non-covalent conjugate of DNA-matrix with neutravidine, dissociation of solid-phase complex "antibody-antigen E.coli O157:H7 - biotin-modified antibody-conjugate" with addition of solution of glycine - HCl pH 2.6, PCR-amplification of DNA-matrix and detection of availability of bacteria E.coli O157:H7 by speed of fluorescent signal increase.EFFECT: method has high specificity and sensitivity of detection and may be used in early diagnostics of haemorrhagic colitis and sanitary-hygienic control over availability of its inducer.5 dwg, 8 ex