Use of Small Molecules to Enhance Mafa Expression in Pancreatic Endocrine Cells

摘要

The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface.

主权项

1. An in vitro cell culture, comprising a population of differentiated pluripotent stem cells expressing markers characteristics of pancreatic endocrine cells, wherein at least 10% of the differentiated cells express insulin, PDX1 NKX6.1, and MAFA.