COMPOSITIONS AND METHODS FOR SINGLE-MOLECULE CONSTRUCTION OF DNA
摘要
The present invention provides for compositions and methods for single-molecule construction of DNA. The present invention provides for a method comprising: (a) providing a reaction chamber comprising a solid support bound to a single starter double-stranded (ds) DNA molecule comprising a free end, (b) introducing one or more extension molecules and one or more enzymes capable of joining a payload region of an extension molecule to the free end of starter dsDNA molecule to the reaction chamber wherein the extension molecule comprises an cleavable linker.
申请公布号
WO2015176070(A1)
申请公布日期
2015.11.19
申请号
WO2015US31444
申请日期
2015.05.18
申请人
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA;ARLOW, DANIEL, H.;HOLTZ, WILLIAM, J.;WEAVER, LANE, J.;KEASLING, JAY, D.
发明人
ARLOW, DANIEL, H.;HOLTZ, WILLIAM, J.;WEAVER, LANE, J.;KEASLING, JAY, D.