SURFACE MEDIATED SYNTHESIS OF POLYNUCLEOTIDES, POLYPEPTIDES AND POLYSACCHARIDES AND RELATED MATERIALS, METHODS AND SYSTEMS

摘要

Surface mediated polymer synthesizing methods and related systems and materials are described where monomers are attached to monomer binding regions on a surface and subsequently form chemical bonds with adjacent monomers on the surface to form linear polymers selected from polynucleotide, polypeptides and polysaccharides.

主权项

1. A method to synthesize a surface-attached linear polymer containing a plurality of constituent monomers of a same or different type, the linear polymer selected from the group consisting of: a polynucleotide containing a plurality of nucleotides of same or different type, a polypeptide containing a plurality of amino acid of same or different type, and a polysaccharide containing a plurality of saccharides of same or different type, the method comprising:
a) attaching monomers of each type of constituent monomer of the linear polymer to attachment sites of a corresponding set of monomer binding regions on a coated surface, to provide attached monomers of each type of constituent monomers of the linear polymer
wherein corresponding sets of monomer binding regions for all types of constituent monomers contained in the polymer, form on the patterned surface a plurality of monomer binding regions in which an attachment site of each monomer binding region of the plurality of monomer binding regions is located at a distance MBD with respect to the attachment site of an adjacent monomer binding region of the plurality of monomer binding regions whereinMBD≅CWem+CWam2 in which CWem is a characteristic width of a monomer bound to said each monomer binding region of the plurality of monomer binding regions and CWam is a characteristic width of a monomer bound to the adjacent monomer binding region of the plurality of monomer binding regions, so that adjacent constituent monomers attached to the plurality of monomer binding regions present corresponding polymerizing functional groups at a distance approximately equal to a corresponding bond length ±50% one with respect to another, and b) performing a coupling reaction between polymerizing functional groups of the attached monomers of each type of constituent monomers of the linear polymer to obtain the surface-attached linear polymer.