Apparatus and Method for Optical Sampling in Miniature Bioprocessing Vessels

摘要

An optical sampling apparatus for miniature-scale bioprocessing vessels includes features for optical interrogation of the bioprocessing vessel contents by means of transmission or transflection spectroscopy. This optical interrogation allows for the determination of quantities and parameters of substances in fluids contained within the bioprocessing vessels during bioprocesses. Multiple such bioprocessing vessels with the optical interrogation features may be mounted in a receiver for conducting multiple bioprocesses simultaneously. A translatable probe may be used to interact with each of the bioprocessing vessels in the receiver.

主权项

1. A disposable bioprocessing vessel for containing a fluid sample, the vessel comprising:
at least one rigid wall; an optical sampling region integral to the rigid wall and comprising a first and second surface to create an optical path between the first and second surfaces within the bioprocessing vessel, said first and second surfaces comprise a polymer at least partially transparent to near-infrared electromagnetic radiation and wherein the first and second surfaces are sufficiently thin to allow near-infrared electromagnetic radiation to pass therethrough, interact with the fluid sample, and be detected outside of the bioprocessing vessel to provide a transmission or transflection measurement of the fluid sample.