| 发明名称 |
METHOD FOR CULTURE OF HUMAN AND MOUSE PROSTATE ORGANOIDS AND USES THEREOF |
| 摘要 |
The invention discloses a methodology for the culture of prostate tissue organoids from mouse and human prostate. |
| 申请公布号 |
US2015329829(A1) |
申请公布日期 |
2015.11.19 |
| 申请号 |
US201514722065 |
申请日期 |
2015.05.26 |
| 申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
SHEN Michael M.;CHUA Chee Wai;LEI Ming |
| 分类号 |
C12N5/071;G01N33/574;C12N5/09;G01N33/50 |
主分类号 |
C12N5/071 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for culturing prostate organoids, the method comprising:
a) obtaining a sample of prostate tissue from a subject; b) dissociating the sample of prostate tissue; c) isolating dissociated prostate epithelial cells from the sample of prostate tissue; and d) culturing the dissociated prostate epithelial cells in a culture medium comprising basal hepatocyte medium, Matrigel, FBS and ROCK inhibitor; wherein the dissociated prostate epithelial cells form prostate organoids in culture. |
| 地址 |
New York NY US |
