COMPOSITIONS, METHODS, AND KITS FOR PREPARING PLASMINOGEN; AND PLASMIN PREPARED THEREFROM

摘要

Compositions and methods for preparing plasminogen, in particular recombinant plasminogen, and compositions and methods of utilizing same for preparing plasmin are provided.

主权项

1. A method for preparing recombinant plasmin, said method comprising:
(a) contacting a composition comprising a recombinant plasminogen having a single kringle domain, a peptide linker, an activation site, and a serine protease domain, wherein said single kringle domain comprises at least 90% identity to kringle 1 or kringle 4 of SEQ ID NO:2; said peptide linker sequence between said single kringle domain and said serine protease domain consists of residues 91-94 of SEQ ID NO:1 with a cation-exchange medium under a cation-exchange condition that is sufficient for said cation-exchange medium to bind said recombinant plasminogen; wherein said cation-exchange medium comprises a sulfopropyl ligand coupled to an agarose support; and (b) converting said recombinant plasminogen to said recombinant plasmin.