METHODS AND COMPOSITIONS FOR ALTERING PHOTOPHYSICAL PROPERTIES OF FLUOROPHORES VIA PROXIMAL QUENCHING

摘要

The invention is directed to fluorophore-containing compositions and configurations wherein proximity between the fluorophore and one or more protective agents (PAs) modifies the lifetime of fluorescent and/or dark states, their frequency of occurrence, and the total lifetime of fluorescence in order to appropriately modify the photophysical characteristics of the fluorophore. The invention is also directed to methods that utilize these compositions and configurations.

主权项

1. A composition comprising a fluorophore having attached thereto at least one protective agent moiety, wherein the at least one protective agent moiety is attached covalently to the fluorophore either directly or via a linker, wherein said protective agent moiety is a moiety that has the ability to alter the photophysical properties of a fluorophore, and is selected from:
(i) chromanol-derived groups having the formula: wherein, in Formula (9), R1, R7, R3 R4 and R5 are independently selected from hydrogen atom and hydrocarbon groups, wherein one of R1, R2, R3, R4 and R5 is a bond connecting the chromanol-derived group to said protective agent; (ii) nitro-substituted aromatic groups having the formula: wherein, in Formula (12), R1, R2, R3, R4, R5, R6, R7, and R8 are independently selected from hydrogen atom and hydrocarbon groups, wherein one of R1, R2, R3, R4, R5, R6, R7 and R8 is a bond connecting the nitro-substituted aromatic group to said protective agent; (ii) conjugated polyene groups; (iv) bicyclic, tricyclic, or higher cyclic ring systems containing at least two, three, or four ring nitrogen atoms; and (v) phenolic derivatives.