摘要 |
A method of determining glycated hemoglobin is provided, by which a ratio of the glycated hemoglobin in a sample can be determined accurately and easily. The ratio of glycated hemoglobin can be determined by hemolyzing whole blood using a non-ionic surfactant; degrading glycated hemoglobin in the hemolyzed whole blood selectively with a protease to give a glycated hemoglobin degradation product; causing a redox reaction between a glycation site of the glycated hemoglobin degradation product and a fructosyl amino acid oxidoreductase; and determining the redox reaction to determine an amount of the glycated hemoglobin; wherein the protease is a metalloproteinase capable of selectively degrading the glycated hemoglobin. |