METHOD FOR A FULLY AUTOMATED MONOCLONAL ANTIBODY-BASED EXTENDED DIFFERENTIAL

摘要

A composition for use in the differentiation or enumeration of hematologic cell types comprises the following components:
A. a first antibody labelled with a first fluorochrome having a first emission spectrum, said first antibody that binds to an antigenic determinant that is differentially expressed on populations of leukocytes and non-leukocytes in a sample;
B. an additional antibody labelled with an additional fluorochrome, wherein said additional antibody binds to an antigenic determinant that is differentially expressed on populations of mature and immature granulocytes or myeloid cells, and wherein said additional fluorochrome has a peak emission spectrum that overlaps said first peak emission spectrum; and
C. a nucleic acid dye which has an emission spectrum that overlaps with at least one of said first emission spectra or said additional emission spectra but wherein the peak emission spectrum of the dye does not overlap with the peak emission spectra of the first and additional fluorochromes. Corresponding methods and kits are also described.