Sensing method and sensing device

摘要

This invention relates to a sensing method and a sensing device for quantifying the concentration of an analyte by using the property that interaction between an analyte and a labeled compound changes fluorescence intensity. A fluorescence sensor is used to acquire fluorescence intensity at predetermined quantification time points. Then, the concentration of an analyte is quantified in accordance with a non-steady concentration quantification law including the relationship between the acquired fluorescence intensity and the time derivative quantity thereof.

主权项

1. A sensing method of quantifying a concentration of analytes based on an intensity of fluorescence, which changes due to an interaction between the analytes and a labeled compound, comprising:
an acquisition step of acquiring the intensity of fluorescence at a predetermined quantification time using a fluorescence sensor; a selection step of selecting a concentration quantification rule from a steady concentration quantification rule in relation to the intensity of fluorescence and a non-steady concentration quantification rule representative of a relationship between the acquired intensity of fluorescence and a time derivative of the intensity of fluorescence at the predetermined quantification time, depending on a time-dependent change in the acquired intensity of fluorescence and/or an ambient temperature; and a quantification step of quantifying the concentration of the analytes according to the selected concentration quantification rule.