Methods and devices for in situ nucleic acid synthesis

摘要

Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.

主权项

1. A method for synthesizing a nucleic acid having a predefined sequence, the method comprising:
a) providing a support comprising a plurality of features, each feature comprising a plurality of single-stranded support-bound oligonucleotides; b) hybridizing a partially double-stranded first oligonucleotide to at least a first support-bound oligonucleotide at a first feature, wherein the first partially double-stranded oligonucleotide comprises a 5′ overhang and a ligatable predetermined addition nucleotide at a 3′ end of the double-stranded portion; c) ligating the partially double-stranded first oligonucleotide to the first support-bound oligonucleotide thereby generating a first ligation product comprising the predetermined addition nucleotide; and d) cleaving the first ligation product thereby generating a first elongated support-bound oligonucleotide comprising the predetermined addition nucleotide.