| 摘要 |
PROBLEM TO BE SOLVED: To provide cell culture of high quality comprising no impurity components resulting from a producing process, which may provide an obstacle for clinical application and a producing method thereof.SOLUTION: In the present invention, there are provided: a producing method of sheet shape cell culture including (i) a process of inoculating cells on a cultivation substrate coated with serum and (ii) a process of generating the sheet shape cell culture by culturing the cells; the sheet shape cell culture produced with the method; the cultivation substrate coated with serum utilized in the method; a producing method and producing kit of the cultivation substrate; and a method for suppressing production of inflammatory cytokine in the cell culture, which method includes (i) a process of inoculating cells on the cultivation substrate coated with serum and (ii) a process of generating cell culture by cultivating the cells with a serum-free medium. |
