| 主权项 |
1. A method for determining a prognosis for ovarian cancer in a subject having ovarian cancer, for diagnosing ovarian cancer in a subject at risk of developing ovarian cancer, or for diagnosing a risk of developing ovarian cancer in a subject, comprising:
a) obtaining a sample from the subject; b) detecting in nucleic acid in the sample from the subject a methylation state of at least three genes, or regulatory regions thereof, wherein the at least three genes include GULP1 and PGP9.5, and at least one additional gene selected from the group consisting of CSGALNACT2, HIC 1, AIM1, APC, PAK3, MCAM, MGMT, KIF1A, CCNA1, ESR1, SSBP2, GSTP1, FKBP4 and VGF, wherein said detecting comprises detecting hybridization of a probe to said nucleic acid and/or to amplification products of said nucleic acid, said probe consisting of the nucleotide sequence of SEQ ID NO: 33 and optionally a label or labels, and wherein said hybridization of said probe indicates that the methylation state of a regulatory region of GULP1 is hypermethylated; c) comparing the methylation state detected in (b) to that of a corresponding normal sample; and d) determining a diagnosis or prognosis based on (c), wherein the methylation state detected in (b) being hypermethylated in at least one of the three genes or regulatory regions thereof as compared to that of the corresponding normal sample is indicative of a poor prognosis in a subject having ovarian cancer, of the presence of ovarian cancer in a subject at risk of developing ovarian cancer, or of a risk of developing ovarian cancer in a subject. |
