Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity

摘要

Compositions and methods comprising polynucleotides and polypeptides having 4-hydroxyphenylpyruvate dioxygenase (HPPD) activity and having insensitivity to an HPPD inhibitor are provided. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the HPPD sequences. Various methods of employing the HPPD sequences are provided. Such methods include, for example, methods for producing an HPPD inhibitor tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. Methods are also provided to identify additional HPPD variants. Further provided are various methods and compositions that allow the various HPPD polypeptides and variant and fragments thereof to be expressed in a chloroplast or transported to a chloroplast.

主权项

1. A nucleic acid construct comprising a polynucleotide,
wherein the polynucleotide comprises a nucleotide sequence encoding an HPPD polypeptide, wherein said polynucleotide encodes a polypeptide comprising an HPPD polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 411 or 438.