| 发明名称 | Directed evolution and in vitro panning of virus vectors | ||
| 摘要 | The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and/or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same. | ||
| 申请公布号 | US9186419(B2) | 申请公布日期 | 2015.11.17 |
| 申请号 | US201414157696 | 申请日期 | 2014.01.17 |
| 申请人 | The University of North Carolina at Chapel Hill | 发明人 | Xiao Xiao;Yang Lin |
| 分类号 | A01N63/00;A61K48/00;C12Q1/70;C07H19/00;C07H21/00;C07K14/005;C12N15/86;A61K38/17;C07K14/47;C12N7/00 | 主分类号 | A01N63/00 |
| 代理机构 | Myers Bigel Sibley & Sajovec, P.A. | 代理人 | Myers Bigel Sibley & Sajovec, P.A. |
| 主权项 | 1. A nucleic acid encoding an AAV capsid, the nucleic acid comprising an AAV capsid coding sequence selected from the group consisting of: (a) the nucleotide sequence of FIG. 3RR (H43) (SEQ ID NO:29); (c) a nucleotide sequence that encodes the AAV capsid encoded by the nucleotide sequence of (a) but that differs from the nucleotide sequence of (a) due to the degeneracy of the genetic code. | ||
| 地址 | Chapel Hill NC US | ||