Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

摘要

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.

主权项

1. A method comprising:
introducing a fluid sample containing a quantity of white blood cells into a channel defined in part by a substrate; forming complexes which include label moieties affixed to at least a portion of the quantity of white blood cells; providing first and second density media in separate chambers defined at least in part by the substrate wherein the first density media has a density different from that of the second density media and wherein the first and second density media have a density lower than a density of the white blood cells and higher than a density of the fluid sample; spinning the substrate to transport the first and second density media from the separate chambers into a detection region defined in part by the substrate; transporting the quantity of white blood cells toward the detection region comprising the layered density media; spinning the substrate such that at least a portion of the quantity of white blood cells is transported through at least a portion of the first or second density media; and detecting signals from the label moieties affixed to the at least a portion of the quantity of white blood cells, including detecting signals from label moieties near an interface of the first and second density media.