体外小肠干细胞培养基及培养方法;CULTURE MEDIA AND CULTURE METHOD FOR INTESTINAL STEM CELLS IN VITRO

摘要

一种用于体外培养小肠干细胞之培养基，包含基础培养液；运铁蛋白；乙醇胺；抗坏血酸、或其盐、或其酯；麸胱甘肽或其盐及亚硒酸钠，其中，该乙醇胺于该培养基中之浓度范围为0.5mM至小于5mM。本发明进一步提供一种体外培养小肠干细胞之方法，其方法包含将所分离之小肠干细胞培养于本发明之培养基中。; transferrin; ethanolamine; ascorbic acid, salt or ester thereof; glutathione or salt thereof and sodium selenite, wherein the concentration of the ethanolamine is in a range of 0.5 mM to less than 5 mM. The present invention further provides a culture method for intestinal stem cells in vitro, and the culture method comprises a step of culturing isolated intestinal stem cells in the culture media of the present invention.