摘要 |
Provided are an RNA cleaving agent, a method for cleaving RNA, a method for constructing an RNA sequencing library, an RNA sequencing library and a sequencing method. The RNA cleaving agent comprises: a biological buffer suitable for maintaining the pH value of the RNA cleaving agent in the range of 7 to 9; and monovalent and divalent metal ions, the biological buffer and the monovalent and divalent metal ions are in effective concentrations. |