METHODS, DEVICES, AND SYSTEMS OF DETECTING MICROORGANISMS

摘要

A rapid, sensitive method of separating and detecting microorganisms from a sample potentially containing microorganisms, such as but not limited to bacteria, fungi, yeast, viruses, and the like. The method relies on separation techniques to separate and concentrate the cells from the sample, together with chemical techniques to amplify the amount of detectable signal from low numbers of cells to provide a rapid and sensitive method of detecting microorganisms. This detection method may utilize: a filtration device; a centrifugation device; a system; a swab device; and kit comprising one or more of the devices and components to perform the present method of separating and detecting microorganisms in a sample potentially containing microorganisms. The sample may be a chemical (e.g., household cleaners), cosmetic, personal care, pharmaceutical, or consumable (e.g., food or beverage) good in its raw material, in-process, and/or finished product states that needs to be tested for any contaminating microorganisms prior to shipment to the consumer.

主权项

1. A method of detecting microorganisms in a sample potentially containing microorganisms, comprising:
a) filtering the sample through a pre-filter for allowing microorganisms to flow through; b) filtering the pre-filter filtrate through a capture filter for retaining microorganisms; c) culturing the microorganisms retained on the capture filter; d) incubating the microorganisms retained on the capture filter with extractant in combination with adenosine diphosphate; e) filtering the lysed cells through the capture filter; f) adding a luciferin/luciferase reagent to the capture filtered lysed cells; and g) detecting microorganisms in the sample using a luminometer.