发明名称 |
FLUORESCENT MOLECULAR PROBES FOR USE IN ASSAYS THAT MEASURE TEST COMPOUND COMPETITIVE BINDING WITH SAM-UTILIZING PROTEINS |
摘要 |
Assay methods may generally comprise forming homogeneous assay mixtures comprising target SAM-utilizing protein, fluorescent detection analyte, and test compound, incubating, and measuring FP or TR-FRET signal emitted in order to determine a measure of test compound-SAM-utilizing protein binding. Assay mixtures comprise a SAM-utilizing protein, and a fluorescent detection analyte that binds with the SAM-utilizing protein in the absence of test compound. Assay mixtures may further comprise a test compound. Assay mixture embodiments may generate FP or TR-FRET signal properties that are a function of the inherent binding interactions of both the test compound and the detection analyte with the SAM-utilizing protein. Fluorescent detection analytes comprise a fluorophore moiety, a covalent linker moiety, and a SAM-utilizing protein ligand moiety and could be utilized in FP or TR-FRET assays to measure test compound binding. |
申请公布号 |
US2015323542(A1) |
申请公布日期 |
2015.11.12 |
申请号 |
US201514801902 |
申请日期 |
2015.07.17 |
申请人 |
Cayman Chemical Company, Incorporated ;The Regents of the University of Michigan |
发明人 |
Barrett Stephen Douglas;Bochar Daniel Austin;Blazer Levi Lynn;Ciske Fred Lawrence;Endres Gregory William;Johnson Jeffrey Keith;Keyes Gregory Scott;Sidhu Ranjinder Singh;Trievel Raymond C.;Collins Margaret Lynn |
分类号 |
G01N33/58;G01N21/64;C07D519/00;C07D473/34;C07D471/04 |
主分类号 |
G01N33/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Ann Arbor MI US |