SINGLE-USE SENSORS IN BIOREACTORS, BIOTECH PURIFICATION AND BIOPROCESSING

摘要

Presteralized manifolds having disposable multifunctional sensors are provided which are designed for sterile packaging and single-use approaches. These manifolds, which have disposable tubing and flexible-wall containers and are adapted to interact with other equipment which can be operated by a controller and a flow-imparting unit, provide automated and accurate delivery or purification of biotechnology fluid. The disposable sensor monitors pH, oxygen and electrical conductivity, alone or in combination, that interact with the controller or are connected to a separate user interface. Disposable sensor components and re-usable components can be dockable with each other.

主权项

1. A manifold system for biotechnology uses, comprising:
a manifold unit which is pre-sterilized and disposable so as to be adapted for single-time usage, including: (a) at least one length of tubing having at least one inlet end portion, at least one outlet end portion, an outside surface, and an inside surface which is sterilized for passage of a biotechnology fluid therethrough, (b) at least one single-use container in fluid communication with said tubing, (c) at least one single-use sensor that senses parameter combinations that include a pH sensing function combined with at least one other sensing function selected from the group consisting of electrical conductivity, oxygen, temperature and combinations thereof, (d) at least one valve remotely operable to engage the length of tubing; (e) a single-use separation component selected from the group consisting of a separation unit, a purification unit, a sterilizing filter and a combination thereof positioned along said length of tubing such that the biotechnology fluid can flow therethrough at a location upstream of said outlet end portion; and a controller which controls operation of said valve, said controller having control logic which dictates the timing of opening and closing of said remotely operable valve responsive to the parameters sensed at the sensor.