ENZYMATIC DETERMINATION OF HBA1C

摘要

The present invention relates to a method for determining the amount of glycated haemoglobin (HbA1c), in which – if required – the erythrocytes in a sample are haemolysed, the haemoglobin that is then released – if required – is contacted with a proteolytic agent and the glycated haemoglobin degradation products obtained in this way or otherwise are quantified. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, for the stabilization of the haemoglobin which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer is present in the haemolysis solution. In the embodiments of the invention in which a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed in accordance with the invention that the latter be stabilized with particular phosphine compounds and/or thio compounds, and in particular embodiments of the invention the requisite proteolytic agent is to be provided in the form of an inactivated protease which is then only reactivated in situ.