USE OF MICROVESICLES IN ANALYZING MUTATIONS

摘要

Microvesicles are small membrane vesicles that either shed or bud off eukarotic cells. Analysis of the nucleic acid content of microvesicles may be useful in detecting the presence or absence of genetic aberrations. This invention discloses novel methods of diagnosing, prognosing, monitoring, or treating a disease, such as cancer, or other medical condition in a subject involving analyzing one or more nucleic acids contained within an isolated microvesicle for the presence or absence of one or more Kras genetic aberrations.

主权项

1. A method for diagnosis or prognosis of a disease or other medical condition associated with a Kras genetic aberration in a subject, comprising isolating one or more microvesicles from a body fluid sample from the subject by filtering the body fluid sample to remove any cell contamination, and analyzing nucleic acid contained within the isolated microvesicles for the presence of a Kras genetic aberration selected from the group consisting of G12A, G12D, G12R, G12C, G12S, G12V, and G13D, wherein the presence of the Kras genetic aberration is indicative of a diagnosis or prognosis of the disease or other medical condition.