发明名称 |
COMPOSITIONS AND METHODS FOR MONITORING TRANSMEMBRANE TRAFFICKING |
摘要 |
Provided herein are compositions and methods for monitoring the movement of analytes and/or cellular components across biological membranes (e.g., cell surface internalization). In particular, reporter constructs are provided, the transmembrane movement of which (e.g., by endocytosis) is monitored by methods described herein. |
申请公布号 |
US2015322482(A1) |
申请公布日期 |
2015.11.12 |
申请号 |
US201514800352 |
申请日期 |
2015.07.15 |
申请人 |
Promega Corporation |
发明人 |
Binkowski Brock F.;Cong Mei;Robers Matthew B. |
分类号 |
C12Q1/66 |
主分类号 |
C12Q1/66 |
代理机构 |
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代理人 |
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主权项 |
1. A method of detecting endocytosis comprising:
a) tethering a reporter element to a cell-surface analyte to create a fusion element; and b) monitoring a detectable signal from said reporter element thereby detecting endocytosis. |
地址 |
Madison WI US |