T1R TASTE RECEPTORS AND GENES ENCODING SAME

摘要

Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular taste stimulus in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed.

主权项

1. An isolated nucleic acid selected from the group consisting of:
(i) a cDNA DNA sequence consisting essentially of a nucleic acid sequence coding for a T1R mammalian G protein-coupled receptor polypeptide active in taste signaling, wherein said nucleic acid sequence consists essentially of a nucleic acid sequence selected from the group consisting of SEQ ID NOs 15 and 20; (ii) a cDNA DNA sequence consisting essentially of a nucleic acid sequence coding for a T1R mammalian G protein-coupled receptor polypeptide have an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, 17, and 21; (iii) a cDNA DNA sequence having at least about 50% identity to a nucleic acid sequence coding for a T1R mammalian G protein-coupled receptor polypeptide, wherein said nucleic acid sequence consists essentially of a nucleic acid sequence selected from the group consisting of SEQ ID NOs 15 and 20; (iv) a cDNA DNA sequence consisting essentially of a nucleic acid sequence coding for a T1R mammalian G protein-coupled receptor polypeptide having an amino acid sequence that is at least about 40% identical to the amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, 17, and 21; (v) a cDNA DNA sequence consisting essentially of a sequence coding for a T1R mammalian G protein-coupled receptor polypeptide comprising a consensus sequence selected from the group consisting of SEQ ID NOs 18 and 19, and sequences having at least about 75% identity to SEQ ID NOs 18 or 19; (vi) a cDNA sequence having the same nucleic acid sequence as the T1R mammalian G protein-coupled receptor polypeptide coding region in the cDNA DNA sequence selected from the group consisting of SEQ ID NOs 15 and 20; (vii) a cDNA sequence coding for a T1R mammalian G protein-coupled receptor polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, 17, and 21; (viii) a cDNA sequence coding for a T1R mammalian G protein-coupled receptor polypeptide comprising a consensus sequence selected from the group consisting of SEQ ID NOs 18 and 19, and sequences having at least about 75% identity to SEQ ID NOs 18 or 19; (ix) a cDNA sequence selected from the group consisting of SEQ ID NOs 3, 13, 16, and 20; (x) a cDNA sequence having at least about 50% sequence identity to the T1R G protein-coupled receptor polypeptide coding region in the cDNA DNA sequence selected from the group consisting of SEQ ID NOs 15 and 20; (xi) a cDNA sequence having at least about 50% sequence identity to a sequence encoding a T1R mammalian G protein-coupled receptor polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, 17, and 21; (xii) a cDNA sequence having at least about 50% identity to a sequence selected from the group consisting of SEQ ID NOs 3, 13, 16, and 20; (xiii) a variant of a nucleotide sequence selected from the group consisting of SEQ ID NOs 3, 13, 15, 16, and 20, containing at least one conservative substitution in a region coding for a T1R G protein-coupled receptor polypeptide active in taste signaling; (xiv) a variant of a nucleotide sequence encoding a T1R G protein-coupled receptor polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs 4, 14, 17, and 21, containing at least one conservative substitution in a T1R mammalian G protein-coupled receptor polypeptide coding region; and (xv) a variant of a cDNA sequence selected from the group consisting of SEQ ID NOs 3, 13, 16, and 20, containing at least one conservative substitution.