| 摘要 |
PROBLEM TO BE SOLVED: To provide cell therapy enabling stable high expression of transgene, in which a stable transgenic cell line is prepared and then implanted in a subject as encapsulated cells.SOLUTION: There is provided a capsule for delivery of a secreted biologically active compound to a subject, where, a. the capsule comprises a biocompatible outer membrane and an inner core; b. the inner core contains cells; c. the cells comprise a heterologous expression construct comprising a structural gene either i. coding for a secretory biologically active polypeptide or, ii. coding for a polypeptide or an siRNA contributing to the generation of a biologically active secretory compound in the cells; and d. the gene is located between two inverted repeats which are substrates for a transposase or other integrases. |
