发明名称 |
RECOMBINANT CLOSTRIDIAL NEUROTOXINS WITH ENHANCED MEMBRANE LOCALIZATION |
摘要 |
This invention relates to novel recombinant clostridial neurotoxins exhibiting increased membrane localization and to methods for the manufacture of such recombinant clostridial neurotoxins. These methods comprise the steps of inserting a nucleic acid sequence coding for a C2 domain into a nucleic acid sequence coding for a parental clostridial neurotoxin and expression of the recombinant nucleic acid sequence comprising the C2 domain in a host cell. The invention further relates to novel recombinant single-chain precursor clostridial neurotoxins used in such methods, nucleic acid sequences encoding such recombinant single-chain precursor clostridial neurotoxins, and pharmaceutical compositions comprising the recombinant clostridial neurotoxin with increased membrane localization. |
申请公布号 |
US2015322118(A1) |
申请公布日期 |
2015.11.12 |
申请号 |
US201314649778 |
申请日期 |
2013.12.05 |
申请人 |
MERZ PHARMA GMBH & CO. KGAA |
发明人 |
GROER Gerhard;FREVERT Jurgen;HOFMANN Fred;SCHMIDT Michael |
分类号 |
C07K14/33;C07K14/47 |
主分类号 |
C07K14/33 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Frankfurt am Main DE |