METHOD FOR SCREENING FOR AUTOPHAGY ACTIVATOR OR INHIBITOR

摘要

The present invention provides a method for screening for an autophagy activator or inhibitor comprising the steps of: (a) making a test material to be analyzed come into contact with cells containing Beclin1 protein; and (b) analyzing the degree of phosphorylation at the 30th serine amino acid residue of the Beclin1 protein. The test material is determined to be an autophagy activator when the phosphorylation of the Beclin1 protein is up-regulated, and the test material is determined to be an autophagy inhibitor when the phosphorylation of the Beclin1 protein is down-regulated. The present invention first establishes, by ULK1, the mechanism of phosphorylation at the 30th serine amino acid residue of Beclin1.