发明名称 |
MULTIPLE EPITOPE DETECTION IN AN FFPE TISSUE SECTION |
摘要 |
A method for labelling a tissue section is provided. In certain embodiments, the method may comprise: (a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (c) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. A kit for performing the method is also provided. |
申请公布号 |
US2015323532(A1) |
申请公布日期 |
2015.11.12 |
申请号 |
US201414273320 |
申请日期 |
2014.05.08 |
申请人 |
Agilent Technologies, Inc. |
发明人 |
Tom-Moy May;Yamada Nazumi Alice |
分类号 |
G01N33/569 |
主分类号 |
G01N33/569 |
代理机构 |
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代理人 |
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主权项 |
1. A method for labelling a tissue section, comprising:
(a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (d) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. |
地址 |
Loveland CO US |