METHODS FOR PRODUCING RETINAL TISSUE AND RETINA-RELATED CELL

摘要

The present invention provides
[1] a method for producing a retinal tissue, including the following steps (1) to (3):
(1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells,
(2) a second step of subjecting the aggregate formed in the first step to floating culture in a serum-free medium containing a basement membrane preparation, and
(3) a third step of subjecting the aggregate cultured in the second step to floating culture in a serum-containing medium;
[2] a method for producing an optic-cup-like structure, including a step of subjecting an aggregate containing the retinal tissue cultured in the step (3) above to floating culture in a serum-free medium or serum-containing medium each containing a substance acting on the Sonic hedgehog signal pathway and a substance acting on the Wnt signal pathway;
[3] a method for producing a retinal pigment epithelium, including a step of subjecting an aggregate containing the retinal tissue cultured in the step (3) above to floating culture in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway (wherein said serum-free medium and serum-containing medium are free of a substance acting on the Sonic hedgehog signal pathway); and
[4] a method for producing a retinal layer-specific neural cell, including bringing a retinal progenitor cell contained in a retinal tissue derived from a primate pluripotent stem cell into contact with a substance inhibiting the Notch signal pathway.