Pharmaceutical compositions for binding sphingosine-1-phosphate

摘要

The present invention relates to compositions that comprise an anti-S1P monoclonal antibody, antibody fragment, or derivative in a carrier, particularly a buffered, hypertonic aqueous solution, as well as to kits containing such compositions, and methods for using such compositions for treatment of diseases and conditions associated with S1P.

主权项

1. A composition comprising a pharmaceutically acceptable carrier and an antibody or an antigen-binding antibody fragment that binds sphingosine-1-phosphate (S1P) under physiological conditions, wherein the composition is hypertonic and the antibody or an antigen-binding antibody fragment comprises at least one heavy chain variable domain and at least one light chain variable domain, wherein:
A. each heavy chain variable domain comprises:
(i) a first sequence of amino acid residues of sequence DHTIH (SEQ ID NO: 4);(ii) a second sequence of amino acid residues selected from the group consisting of AISPRHDITKYNEMFRG (SEQ ID NO: 6); and(iii) a third sequence of amino acid residues of sequence GGFYGSTIWFDF (SEQ ID NO: 5); and B. each light chain variable domain comprises the following:
(i) a first sequence of amino acid residues of sequence ITTTDIDDDMN (SEQ ID NO: 1);(ii) a second sequence of amino acid residues of sequence EGNILRP (SEQ ID NO: 2); and(iii) a third sequence of amino acid residues of sequence LQSDNLPFT (SEQ ID NO: 3),wherein the composition comprises a buffered hypertonic solution having a pH between about pH 5.5 and about pH 6.5 and comprising sodium chloride in a concentration of at least about 250mM.