Optical tissue sectioning using full field optical coherence tomography

摘要

According to a first aspect, the invention relates to a multimodal optical sectioning microscope (200, 400, 600) for full-field imaging of a volumic and scattering sample comprising: —a full-field OCT system for providing an image of a first section in depth of the sample comprising an illumination sub-system (201, 401, 601) and a full-field imaging interferometer with a detection sub system (208, 408, 608) and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises a microscope objective (203, 403, 603), —a supplementary full-field optical sectioning imaging system for providing a fluorescent image of a second section in depth of said sample comprising a structured illumination microscope with an illumination sub system (623), means (421, 422) for generating at the focal plane of said microscope objective of said full-field imaging interferometer a variable spatial pattern illumination and a detection sub system (624), optically conjugated with said focal plane of the microscope objective.

主权项

1. A multimodal optical sectioning microscope for full-field imaging of a volumic and scattering sample comprising:
a full-field Optical Coherence Tomography (OCT) system for providing an image of a first section in depth of the sample comprising a first illumination sub-system and a full-field imaging interferometer, wherein the full-field imaging interferometer comprises: a first beamsplitter for defining a reference arm and an object arm for receiving the sample, wherein the reference arm comprises a reference mirror and the object arm comprises a microscope objective, a detection sub system, and an optical conjugation device for optically conjugating the sample and said detection sub system, wherein said optical conjugation device comprises the microscope objective; a supplementary full-field optical sectioning imaging system for providing a fluorescent image of a second section in depth of said sample comprising a structured illumination microscope, wherein the structured illumination microscope comprises: a second illumination sub system separate from the first illumination sub system, an imaging channel comprises the microscope objective of the full-field imaging interferometer and means for generating at the focal plane of said microscope objective a variable spatial pattern illumination, wherein the imaging channel is optically coupled with the object arm of the full-field imaging interferometer using the first beamplitter, and a detection sub system, optically conjugated with said focal plane of the microscope objective; and a second dichroic beamsplitter, wherein light emitted by the first and second illumination sub systems is combined in the object arm using the first beamsplitter and the second dichroic beamsplitter.