Mass spectrometry imaging method for detecting and quantifying a target molecule in a tissue sample

摘要

The invention relates to a method for identifying and quantifying by mass spectrometry imaging at least one target analyte in a tissue sample, comprising the following steps:
a) depositing the tissue sample to be analyzed on a support;b) analyzing the sample by mass spectrometry imaging, so as to obtain the mass spectrum of the target analyte in said tissue sample;c) weighting a signal associated with the mass spectrum of the target analyte in said tissue sample by a tissue extinction coefficient (TEC) specific to the target analyte and to the tissue sample which is obtained by using a control tissue sample; andd) using the weighted signal of the target analyte to determine the quantity of target analyte in the tissue sample.

主权项

1. A method for detecting the distribution and quantifying by mass spectrometry imaging at least one target analyte in a tissue sample, comprising the following steps:
a) depositing the tissue sample containing at least one target analyte on a support for mass spectrometry imaging; b) analyzing the tissue sample by mass spectrometry imaging, so as to obtain a mass spectrum signal of said at least one target analyte in said tissue sample; c) weighting the signal associated with the mass spectrum of said at least one target analyte in said tissue sample by a tissue extinction coefficient (TEC) specific to each of said at least one target analyte and to the tissue sample, wherein the TEC specific to each of said at least one target analyte is obtained according to the following steps:
depositing a control tissue sample on a support for mass spectrometry imaging;adding a known concentration of at least one target analyte to a mass spectrometry imaging matrix;depositing the mixture of said matrix and said at least one target analyte of known concentration on the control tissue sample and said support;analyzing the control tissue sample by mass spectrometry imaging, so as to obtain a mass spectrum signal of said at least one target analyte both in the control tissue sample and on said support;and calculating the TEC according to the formula:TEC=Signal⁢⁢of⁢⁢each⁢⁢of⁢⁢said⁢⁢atleast⁢⁢one⁢⁢target⁢⁢analyte⁢⁢on⁢⁢the⁢⁢supportSignal⁢⁢of⁢⁢each⁢⁢of⁢⁢said⁢⁢atleast⁢⁢one⁢⁢target⁢⁢analyte⁢⁢in⁢⁢the⁢⁢control⁢⁢tissue⁢⁢sampleor⁢⁢inversely⁢:TEC=Signal⁢⁢of⁢⁢each⁢⁢of⁢⁢said⁢⁢atleast⁢⁢one⁢⁢target⁢⁢analyte⁢⁢in⁢⁢said⁢⁢control⁢⁢tissue⁢⁢sampleSignal⁢⁢of⁢⁢each⁢⁢of⁢⁢said⁢⁢atleast⁢⁢one⁢⁢target⁢⁢analyte⁢⁢on⁢⁢the⁢⁢support  wherein said target analyte is optionally isotopically labeled; and d) using each weighted signal of each of said at least one target analyte to determine the quantity and distribution of each of said at least one target analyte in the tissue sample.