Materials and methods directed to asparagine synthetase and asparaginase therapies

摘要

Materials and Methods for use in treating cell proliferative disorders related to asparagine metabolism are provided. Cell proliferative disorders include such cancers as forms of leukemia, ovarian cancers, melanomas, renal cancers, breast cancers, brain cancers, and other cancers. Methods include the use of RNA interference targeted at asparagine synthetase to enhance the efficacy of L-asparaginase therapies.

主权项

1. A kit comprising (a) at least one probe for detecting a level of expression of an endogenous asparagine synthetase (ASNS) gene protein product in a sample from a human subject and (h) at least one standard comprising at least one human cell expressing a level of an ASNS protein, wherein the level of expression in the sample is associated with an efficacy of L-asparaginase (L-ASP) in decreasing cell proliferation and the efficacy is identified based on comparison of the level of expression detected by the probe in the sample to the level of expression detected by the probe in the standard, wherein a higher level of expression in the sample compared to the standard indicates a lower efficacy of L-ASP in decreasing cell proliferation in the sample compared to the standard and wherein a lower level of expression in the sample compared to the standard indicates a higher efficacy of L-ASP in decreasing cell proliferation in the sample compared to the standard, wherein the probe is a non-human antibody or an antigen binding fragment thereof that specifically binds to an epitope of an ASNS protein.