Kits for and methods of differential staining of cervical cancer cells and/or tissues

摘要

Provided are methods and kits for staining cervical cell sample by contacting the cervical cell sample with a Ficus elastics plant extract, staining the cervical cell sample with New Fuchsin, and staining the cervical cell sample with Light Green or Fast Green. Also provided are method of diagnosing a pre-malignant or a malignant cervical tumor in a subject, by staining the cervical cell sample and identifying at least one cervical cell having a red cytoplasm above a pre-determined threshold, wherein presence of the at least one cervical cell having the red cytoplasm above the pre-determined threshold is indicative of a non- or less-differentiated cell as compared to a normal cervical cell, thereby diagnosing the pre-malignant or a malignant cervical tumor in the subject.

主权项

1. A method for differential staining a liquid-based cervical monolayer cell sample, the method comprising the steps of:
(i) incubating a liquid-based cervical monolayer cell sample devoid of blood cells with trichloroacetic acid; (ii) contacting the cervical cell sample with a Ficus elastics plant extract, (iii) staining the cervical cell sample with new fuchsin, (iv) staining the cervical cell sample with light green or fast green, and (v) staining the cervical cell sample with an agent which differentially stains mucin in the cervical cell sample in a color distinguishable from the color obtained by staining with new fuchsin, light green and fast green to differentially stain the cervical cell sample.