| 发明名称 | Method for isolating osteopontin using concentrated feeds | ||
| 摘要 | The present invention pertains to a method for isolating osteopontin from milk-derived feeds having a high concentration of protein. Particularly, the present method involves the use of a narrow window of pH and specific conductance of the milk-derived feed, which surprisingly has proven to provide a very efficient isolation of osteopontin from chemically complex feeds. | ||
| 申请公布号 | US9181325(B2) | 申请公布日期 | 2015.11.10 |
| 申请号 | US201214002614 | 申请日期 | 2012.03.05 |
| 申请人 | ARLA FOODS AMBA | 发明人 | Bertelsen Hans;Wejse Peter Langborg;Trugvason Trine |
| 分类号 | C07K14/78;C07K14/52 | 主分类号 | C07K14/78 |
| 代理机构 | Hershkovitz & Associates, PLLC | 代理人 | Hershkovitz & Associates, PLLC ;Hershkovitz Abe;Aksman Stanislaus |
| 主权项 | 1. A method for isolating osteopontin from a milk-derived feed, the method comprising the steps of: a) providing a milk-derived feed comprising osteopontin, said milk-derived feed having a pH in the range of pH 3.6-6.5 at 25 degrees C. and a specific conductance in the range of 4-10 mS/cm at 25 degrees C., and wherein the milk-derived feed comprises a total amount of protein in the range of 50-250 g/L milk-derived feed, b) subjecting said milk-derived feed to anion exchange chromatography, which includes contacting the milk-derived feed with an anion exchange medium, c) optionally, washing the anion exchange medium, and d) recovering the protein bound to the anion exchange medium, thereby obtaining a composition comprising isolated osteopontin. | ||
| 地址 | DK | ||