Method and device for separating fractions of a mixture

摘要

The invention concerns a method for separating fractions of a mixture to be separated, in a device (1) comprising: several chromatography columns (21, 22, 23,) mounted in series; an open separation loop comprising in input a point for injecting (6) eluent in one of the columns and in output a point for drawing (8) a fraction of the mixture. The method includes the following steps: batch injection of the mixture to be separated in the open separation loop (4); collecting at least two fractions; offsetting at least one column of the eluent injecting and fraction drawing points (6, 8) of the separation loop (4). The invention also concerns a device for separating fractions of a mixture to be separated.

主权项

1. A method for separating fractions of a mixture to be separated, in a device (1) having:
several chromatography columns (21, 22, 23, etc.) mounted in series, a single separation zone constituted by a constantly open separation loop comprising, at the inlet of the open separation loop an eluent-injection point (6) for injecting eluent into one of the columns and, at the outlet of the open separation loop, a draw-off point (8) for withdrawal of a fraction of the mixture, with no other fluid circulating between the inlet and the outlet of the open separation loop, the method comprising: a mixture injection stage for discontinuous injection of the mixture to be separated into the open separation loop (4), a collection stage for collection of at least two fractions, and a shifting stage for shifting by at least one column the eluent-injection point (6) and the fraction draw-off point (8) of the separation loop (4), wherein (a) during the shifting stage, the eluent-injection and draw-off points of the separation loop are shifted at different times, or (b) said device further comprises at least one other loop, or (c) in which the eluent-injection and draw-off points of the separation loop are not contiguous.