| 摘要 |
FIELD: medicine, pharmaceutics.SUBSTANCE: invention relates to biotechnologies, namely to a method of purification or enrichment of blood-coagulation factor FVIII with the application of chromatography. The method includes provision of a fraction, which contains recombinant FVIII, in a water solution with high ionic power, where the water solution contains recombinant FVIII in a solution with the high concentration of salt, corresponding to conductivity in the range from approximately 25 to approximately 200 mC/cm at 25°C, bringing the fraction, which contains recombinant FVIII, in contact with multimodal resin, which represents Capto Adhere or Capto MMC. The fractions which contain recombinant FVIII are eluted with a water eluting buffer, which contains, at least, one amino acid, which is positively charged at a pH value of 6-8, where the amino acid, which is positively charged at the pH value of 6-8, is selected from the group of amino groups, including amino acids, such as lysine, arginine, histidine and their combinations, in the concentration over 0.4 M.EFFECT: invention makes it possible to obtain the purified or enriched factor of blood-coagulation FVIII with high output.14 cl, 8 dwg, 22 tbl, 13 ex |
