Highly sensitive system and method for analysis of troponin

摘要

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

主权项

1. A method for determining a characteristic of cardiovascular health, comprising:
(a) determining a threshold concentration of at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) in blood, serum or plasma in a healthy population, wherein the threshold concentration is less than about 10 pg/ml; (b) measuring the concentration of the at least one of cTnI and cTnT in a blood, serum or plasma sample from the subject in a single molecule counting assay having a limit of detection for the at least one of cTnI and cTnT of less than 3 pg/mL and a coefficient of variation of less than 10%, wherein the assay comprises contacting the sample with an antibody specific for the at least one of cTnI and cTnT and determining the amount of specific binding between the antibody and the at least one of cTnI and cTnT, (c) determining a characteristic of cardiovascular health in the individual when the concentration of the at least one of cTnI and cTnT in the sample is greater than concentration of the at least one of cTnI and cTnT in the threshold concentration.