METHODS AND COMPOSITIONS OF PROTEIN ANTIGENS FOR THE DIAGNOSIS AND TREATMENT OF LEPTOSPIROSIS

摘要

Novel immunodominant antigenic proteins and peptides associated with associated with leptospirosis were identified using a proteome array based on expression of ORFs from a Leptospira genome. Compositions, methods, and uses of such antigenic proteins and peptides in the diagnosis and staging of leptospirosis infection and in compositions, methods, and uses of such antigenic is proteins and peptides in prophylactic and therapeutic vaccines are disclosed.

主权项

1. An antigen composition comprising:
a plurality of antibody reactive antigens associated with or formulated with a carrier, wherein at least two of the antibody reactive antigens have quantified and known relative antibody reactivities with respect to sera of a population affected by leptospirosis, and wherein the at least two of the antibody reactive antigens have a known association with a disease parameter; wherein the plurality of antibody reactive antigens are selected from the group consisting of: LIC1 1352, LIC12544, LIC12631, LIC10464-s1, LIC1 1335, LIC20301, LIC10486, LIC10191, LIC1 1389, LIC1 1437, LIC20087, LIC10623, LIC10998, LIC10215, LIC1 1271, LIC10491-s1, LIC13050, LIC1 1210, LIC10524, LIC1 1456, LIC12476, LIC1 1570, LIC13244, LIC13238, LIC1 1885, LIC1 1008, LIC13242, LIC1 1336, LIC20250, LIC10525, LIC10464-s2.1, LIC20118, Cop LigAU (unique):Repeats A7′-13, CopLigBU (unique):Repeats B7′-12, CopLigB:Repeats 1-16, nt154-173 and antibody reactive fragments thereof.