SECONDARY ANTIBODY DETECTED EPITOPE AND PREPARATION OF SAME

摘要

A method of preparing an epitope for a secondary antibody detected protein tag, comprising: constructing a expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag comprising at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell. Further, a method for constructing a protein molecular weight marker ladder, comprising constructing a plurality of recombinant protein tags with different molecular weights, wherein each the recombinant protein tag comprises at least one epitope of primary antibody. Besides, a secondary antibody detected protein tag comprises at least two peptide sequences of epitopes selected from primary antibodies of at least two different species.

主权项

1. A method of preparing a secondary antibody detected protein tag, comprising:
constructing an expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag, wherein the secondary antibody detected protein tag comprises at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the expressing vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell.