发明名称 |
METHOD FOR ISOLATING POLY(A) NUCLEIC ACIDS |
摘要 |
<p>The present invention pertains inter alia to a method for isolating poly(A) nucleic acids having a single stranded poly(A) stretch from a nucleic acid containing sample comprising: (a) providing a hybridization composition comprising: i) a nucleic acid containing sample; ii) a hybridization solution comprising: aa. a sodium salt; bb. a quaternary ammonium salt; wherein the components of the hybridization solution can be added as single solution to the sample or may be added separately in any order to the sample; iii) a capture probe capable of hybridizing to the poly(A) stretch of the poly(A) nucleic acids; and incubating said hybridization composition under conditions to form nucleic acid-hybrids between the poly(A) nucleic acids and the capture probe; (b) separating the formed hybrids from the remaining sample. The method is in particular suitable for efficiently isolating poly(A) RNA from various samples while avoiding carry-over of unwanted non-poly(A) nucleic acids such as rRNA. Also provided are advantageous further methods, hybridization solutions and kits.</p> |
申请公布号 |
WO2015165859(A1) |
申请公布日期 |
2015.11.05 |
申请号 |
WO2015EP59117 |
申请日期 |
2015.04.28 |
申请人 |
QIAGEN GMBH |
发明人 |
CHRISTOFFEL, GABRIELE;SCHLUMPBERGER, MARTIN;O'NEIL, DOMINIC |
分类号 |
C12N15/09 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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