主权项 |
1. A method of reducing an α, β unsaturated compound, the method comprising:
(a) providing: (i) an α,β unsaturated compound selected from the group consisting of an α,β unsaturated ketone, an α,β unsaturated aldehyde, an α,β unsaturated nitrile, and an α,β unsaturated ester; and (ii) an enone reductase polypeptide comprising an amino acid sequence that is at least 80% identical to a SEQ ID NO:8, wherein the residue corresponding to X251 is A, C, R, E, D, W, Y, R, S, V, G, L, or I; and (b) contacting the α, β unsaturated compound with the enone reductase polypeptide under reaction conditions suitable for conversion of the α, β unsaturated compound to the corresponding saturated product compound selected from the group consisting of a saturated ketone, a saturated aldehyde, a saturated nitrile, and a saturated ester. |